ePoster listing and sessions

Topic: ESOPRS 2021 ePoster sessions
Time: Sep 17, 2021 16:00 Amsterdam, Berlin, Rome, Stockholm, Vienna, 15:00 London

 

 

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What is the best reconstructive option to replace the tarsus? A biomechanical, histological and optical study comparing 5 grafts.

Author: ARNAUD MARTEL
ePoster Number: 217


Purpose

Posterior lamellar reconstruction following eyelid tumor excision is challenging and not consensual. Tarsal grafting is the best option but is available in limited amount. The aim of this study was to compare the biomechanical, histological and optical properties of 5 commonly used grafts to determine the best reconstructive option to replace the tarsus.


Methods

This study was conducted in Nice University hospital between June 2019 and June 2020. Five tissues (tarsus, conchal cartilage, sclera, hard palate mucous membrane [HPM] and dermis) were taken from 5 fresh frozen cadavers for posterior lamellar reconstruction. Biomechanical properties were assessed by tractometry. Collagen and elastin fibers were analyzed histologically and characterized optically with Second Harmonic Generation (SHG) imaging.


Results

The mean Young’s Modulus was 8.92 (2.90-22.90), 1.05 (0.39-1.76), 8.72 (2.0-23.50), 2.57 (0.41-4.35) and 1.44 (0.71-2.30) MPa and the mean tensile strength was 3 (1.70-6.88), 0.54 (0.13-0.79), 2.87 (1.23-5.40), 1.4 (0.21-2.40) and 1.0 (0.46-1.43) MPa for the tarsus, conchal cartilage, sclera, HPM and dermis, respectively. The HPM had the elastin fiber/collagen fiber ratio closest to that of the tarsus. The average SHG intensity was 221 (165-362), 182 (35-259), 369 (206-533), 108 (34-208) and 244 (195-388) a.u for the tarsus, conchal cartilage, sclera, HPM and dermis, respectively. The HPM and dermis had the collagen fiber size and orientation closest to those of the tarsus, respectively.


Conclusion

By assigning 2 points to each characteristic (biomechanical, histological, and optical), the HPM and the sclera appeared to be the best grafts to replace the tarsus.


Additional Authors

First nameLast nameBase Hospital / Institution
sebastienSCHAUBSorbonne University, CNRS, Developmental Biology Laboratory (LBDV), Quai de la Darse, 06234 Villefranche-sur-Mer Cedex, France
StephanieBAILLIFOphthalmology department, University Hospital of Nice, 30 Voie Romaine, 06000 Nice, France
SandraLASSALLELaboratory of Clinical and Experimental Pathology, Côte d’Azur University, FHU OncoAge, Pasteur Hospital, 30 Avenue de la voie Romaine, 06000 Nice, France.
JacquesLAGIEROphthalmology department, University Hospital of Nice, 30 Voie Romaine, 06000 Nice, France
PaulMARTELSterlab laboratory, 2720 Chemin saint Bernard, 06220 Vallauris, France.
KenMARTINIOphthalmology department, University Hospital of Nice, 30 Voie Romaine, 06000 Nice, France

Abstract ID: 21-107