Tear fluid alpha-2-macroglobuline activity in lacrimal gland tumors
Author: Anush Amiryan
Base Hospital / Institution: Helmholtz National Medical Research Center of Eye Diseases
ePoster presentation
Abstract ID: 24-312
Purpose
Lacrimal gland tumors are characterized by similar symptoms, the diagnosis of which is based on a set of clinical and instrumental data. Alpha-2-macroglobulin (α2-MG) is a multifunctional protein of the acute phase of inflammation, which is capable of inhibiting a wide range of proteinases, link a number of growth factors, cytokines, ions and lipid factors. Since it is known that any tumor is accompanied by an inflammatory reaction, our goal was to study the activity of α2-MG in the tear fluid in patients with lacrimal tumors.
Methods
The study included 13 patients (26 orbits) with lacrimal gland tumors. Tear samples were collected from both eyes before surgery using filter paper. The activity of α2-MG was determined by the enzymatic method with the specific substrate N-benzoyl-DL-arginine-p-nitroanilide. The control group included healthy orbits of these patients, with the exception of bilateral pathology, which was observed in 2 cases. Based on the results of histological examination of the surgical material, orbital lymphoma was diagnosed in 5 cases, pleomorphic adenoma in 5 cases, and lacrimal gland carcinoma in 3 patients.
Results
We found that in epithelial tumors of the lacrimal gland – pleomorphic adenoma and carcinoma, there was an increase in α2-MG activity in the tear fluid on the affected side: 8.87±3.8 nmol/min×ml in pleomorphic adenoma and 11.98±3.9 nmol/min×ml in carcinomas, respectively, compared with the paired side. Thus, in 2 of 3 cases of lacrimal gland carcinomas, there was a increasing tendency in α2-MG activity also on the healthy side (9.18±6.6 nmol/min×ml), while in the control group it was 5.92±2.6 nmol/min×ml. In lacrimal gland lymphomas no significant differences in the activity of α2-MG in the tear fluid in the affected orbit and on the healthy side were not detected (4.64 ± 1.5 nmol/min×ml and 4.44 ± 3.5 nmol/min×ml, respectively).
Conclusion
Thus, based on the results, we can express the opinion, that determining the activity of α2-MG in the tear fluid may be useful in understanding some aspects of the pathogenesis of lacrimal gland tumors and play a role in non-invasive diagnosis of tumors.
Additional Authors
| First name | Last name | Base Hospital / Institution |
|---|---|---|
| Svetlana | Saakayn | Helmholtz National Medical Research Center of Eye Diseases |
| Mariya | Gusakova | Helmholtz National Medical Research Center of Eye Diseases |
| Olga | Beznos | Helmholtz National Medical Research Center of Eye Diseases |
| Tatyana | Pavlenko | Helmholtz National Medical Research Center of Eye Diseases |
