Topic: ESOPRS 2021 ePoster sessions
Time: Sep 17, 2021 16:00 Amsterdam, Berlin, Rome, Stockholm, Vienna, 15:00 London
(plain text version here)
Photoacoustic imaging of periocular skin cancer – unique spectral signatures of malignant melanoma, basal and squamous cell carcinoma
Author: Magne Tordengren
ePoster Number: 191
Purpose
When removing periocular skin tumors, it is important to spare healthy tissue. Photoacoustic (PA) imaging is a non-invasive biomedical imaging modality with potential for intraoperative micrographic control of surgical margins. This is the first study to assess the feasibility of PA imaging for detection of periocular skin cancer.
Methods
Eleven patients underwent surgical excision of periocular skin cancer, one of which was a malignant melanoma (MM), eight were basal cell carcinomas (BCCs) and two squamous cell carcinomas (SCCs). Six tumors were located in the eyelid, and five in periocular skin. The excised samples as well as healthy eyelid samples were scanned with PA imaging postoperatively, using 59 wavelengths in the range 680 – 970 nm, to generate 3D multispectral images. Spectral unmixing was performed using endmember spectra for oxygenated and deoxygenated Hb, melanin, and collagen, to identify the chromophore composition of tumors and healthy eyelid tissue. After PA scanning, the tumor samples were examined histopathologically using standard hematoxylin and eosin (H&E) and immunohistochemical staining.
Results
The PA spectra of healthy eyelid tissue were dominated by melanin in the skin, oxygenated and deoxygenated hemoglobin in the orbicularis oculi muscle, and collagen in the tarsal plate. Multiwavelength 3D scanning provided spectral information on the three tumor types. The spectrum from the MM was primarily reconstructed by the endmember melanin, while the SCCs showed contributions primarily from melanin, but also HbR and collagen. BCCs showed contributions from all four endmembers with a predominance of HbO2 and HbR.
Conclusion
PA imaging may be used to distinguish different kinds of periocular skin tumors, paving the way for future intraoperative micrographic control.
Additional Authors
| First name | Last name | Base Hospital / Institution |
|---|---|---|
| Jenny | Hult | Department of Clinical Sciences, Skåne University Hospital |
| Aboma | Merdasa | Department of Clinical Sciences, Skåne University Hospital |
| John | Albinsson | Department of Clinical Sciences, Skåne University Hospital |
| Agnes | Pekar-Lukacs | Department of Pathology, Skåne University Hospital |
| Bodil | Gesslein | Department of Clinical Sciences, Skåne University Hospital |
| Ulf | Dahlstrand | Department of Clinical Sciences, Skåne University Hospital |
| Karl | Engelsberg | Department of Clinical Sciences, Skåne University Hospital |
| Johanna | Berggren | Department of Clinical Sciences, Skåne University Hospital |
| Magnus | Cinthio | Department of Biomedical Engineering, Faculty of Engineering, Lund University |
| Rafi | Sheikh | Department of Clinical Sciences, Skåne University Hospital |
| Malin | Malmsjö | Department of Clinical Sciences, Skåne University Hospital |
Abstract ID: 21-153