Topic: ESOPRS 2021 ePoster sessions
Time: Sep 17, 2021 16:00 Amsterdam, Berlin, Rome, Stockholm, Vienna, 15:00 London
(plain text version here)
Evaluation of Carbonic Anhydrase IX and Serum Antioxidant Enzymes in Patients with Periocular Basal Cell Carcinoma
Author: Nilay Yuksel
ePoster Number: 202,00
Purpose
To investigate the immunohistochemical (IHC) expression of Carbonic Anhydrase IX (CA-IX) in periocular basal cell carcinoma (BCC), as well as the levels of CA-IX and key antioxidant enzymes in serum.
Methods
Fifteen patients with periocular BCC were prospectively included. Immunohistochemical analysis of CA-IX expression was performed on tumor tissue excised with clear surgical margins of at least 2 mm. Serum levels of Superoxide Dismutase 1 (SOD1), Catalase (CAT), Glutathione Peroxidase 1 (GPX1), and CA-IX were measured preoperatively and one month postoperatively using the ELISA method. A control group consisting of 18 age- and gender-matched individuals without systemic disease who underwent upper eyelid blepharoplasty was also included.
Results
In the BCC group, preoperative serum CA-IX levels were significantly higher, while serum GPX1, CAT, and SOD1 levels were significantly lower compared to the control group (p = 0.001). CA-IX staining was positive in 11 out of 15 BCC patients (73%) based on IHC analysis. One month after surgery, an increase in SOD1 and GPX1 levels and a decrease in CA-IX levels were observed in BCC patients (p < 0.05). A negative and statistically significant correlation was found between preoperative serum CA-IX levels and CAT, SOD1, and GPX1 levels (p < 0.05). Similarly, a negative and significant correlation was observed between postoperative serum CA-IX and GPX1 levels (p < 0.05).
Conclusion
The results of our study indicate that CA-IX is highly present in periocular BCC tissues. The higher serum levels of CA-IX in BCC patients compared to healthy individuals suggest that this enzyme should be considered not only as localized in tumor tissue but also as a systemic marker. The lower levels of antioxidant enzymes detected in BCC patients may be responsible for the etiology as an indicator of possible systemic oxidative stress in these patients.
Additional Authors
| First name | Last name | Base Hospital / Institution |
|---|---|---|
| Ayse Nilay | Ozgur-Sari | University of Health Sciences, Department of Ophthalmology, Ankara Bilkent City Hospital, Ankara, Turkiye |
| Haci Ahmet | Kilic | Department of Medical Biology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkiye. |
| Ozen | Ozensoy-Guler | Department of Medical Biology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkiye. |
| Hatice | Yavas | Department of Pathology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkiye. |
| Fazli | Erdogan | Department of Pathology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkiye. |
Abstract ID: 25-240
